LeGene SB-Green One-Step qRT-PCR Master Mix (2X)
LeGene Biosciences는 미국 샌디에이고에 위치한 회사로 효소 연구, 개발 및 제조를 전문으로 하는 생명공학 회사입니다.
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Catalog No. 6460-05: LeGene SB-Green One-Step qRT-PCR Master Mix (2X), Size: 50 X 50 ul reactions
Catalog No. 6460-20: LeGene SB-Green One-Step qRT-PCR Master Mix (2X), Size: 200 X 50 ul reactions
Catalog No. 6460-50: LeGene SB-Green One-Step qRT-PCR Master Mix (2X), Size: 500 X 50 ul reactions
Catalog No. 6461-05: LeGene SB-Green One-Step qRT-PCR Master Mix, Low ROX (2X), Size: 50 X 50 ul reactions
Catalog No. 6461-20: LeGene SB-Green One-Step qRT-PCR Master Mix, Low ROX (2X), Size: 200 X 50 ul reactions
Catalog No. 6461-50: LeGene SB-Green One-Step qRT-PCR Master Mix, Low ROX (2X), Size: 500 X 50 ul reactions
Catalog No. 6462-05: LeGene SB-Green One-Step qRT-PCR Master Mix, ROX (2X), Size: 50 X 50 ul reactions
Catalog No. 6462-20: LeGene SB-Green One-Step qRT-PCR Master Mix, ROX (2X), Size: 200 X 50 ul reactions
Catalog No. 6462-50: LeGene SB-Green One-Step qRT-PCR Master Mix, ROX (2X), Size: 500 X 50 ul reactions
Features
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Highly convenient and easy-to-use one tube 2X Master Mix solution
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Highly convenient and easy-to-use one tube 2X Master Mix solution
Highly convenient and easy-to-use one tube 2X Master Mix solution
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An engineered RnaUsScript RT: high yield and broad range of RT reaction temperature (40-55oC)
An engineered RnaUsScript RT: high yield and broad range of RT reaction temperature (40-55oC)
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Broad dynamic range of detection: over six orders of dynamic range
Broad dynamic range of detection: over six orders of dynamic range
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High sensitivity and specificity: detect as low as 0.1pg of total RNA
High sensitivity and specificity: detect as low as 0.1pg of total RNA
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Antibody based hot start PCR system
Antibody based hot start PCR system
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Fully optimized qRT-PCR buffer: robustness and reliability
Fully optimized qRT-PCR buffer: robustness and reliability
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Three choice of master mixes to accommodate the real-time detection systems: No ROX, Low ROX, ROX
Three choice of master mixes to accommodate the real-time detection systems: No ROX, Low ROX, ROX
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Optimally formulated for SYBR-based everyday qRT-PCR
Optimally formulated for SYBR-based everyday qRT-PCR
Description
LeGene SB-Green One-Step qRT-PCR Master Mix Rox is a 2X concentrated formulation mix. It is designed for the reverse transcription (RT) and polymerase chain reaction (PCR) amplification of a specific target RNA from either total RNA or mRNA in a single tube for the detection and quantification of RNA using real-time detection instruments.
The proprietary 2X qRT-PCR Master Mix contains an engineered RnaUsScript Reverse Transcriptase (RNaseH minus), DnaUs Hot Start Taq DNA polymerase, dNTPs, Mg2+, enhancer, stabilizer and an optimal concentration of ROX for instruments that require ROX for a reference signal. Please consult our website and Instrument Compatibility section below for the correct product for your real-time PCR system.
DnaUs Hot Start Taq DNA polymerase is an antibody-inactivated hot-start enzyme designed to block polymerase activity between ambient to RT reaction temperature. RnaUsScript RT enzyme can synthesize cDNA at a temperature range of 40-55oC. Once the PCR step reaches denaturation temperature (95oC), Taq DNA polymerase activity is restored and the resulting PCR exhibits higher sensitivity, specificity and yield.
The system enables highly sensitive detection from as few as 1 copy of a target gene, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 μg of total RNA. Sufficient reagents are provided for 50, 200 or 500 amplification reactions of 50 μl each.
LeGene Biosciences는 미국 샌디에이고에 위치한 회사로 효소 연구, 개발 및 제조를 전문으로 하는 생명...
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